A new report has blamed the deaths on the Assad regime for its continued blockade of the town, and accused the United Nations of failing to provide urgently needed aid.
In January, horrific images emerged of deathly skinny, emaciated children in Madaya.
The town of 40,000 has been surrounded by pro-Assad forces since July 2015, with barely anyone or anything allowed in or out.
Since the issue was brought to the UN six months ago, 27 more people in Madaya have reportedly died of starvation or malnourishment.
According to the new report, compiled by the Syrian American Medical Society and Physicians for Human Rights, Madaya has not received aid for the last two months.
It details a timeline of how the Assad regime continued to block aid for no reason but to starve civilians, and says the UN continued to fail to deliver aid to these areas.
Lead author Elise Baker said the report found evidence that the Assad regime removed items from a humanitarian convoy to Madaya in February, including kits for treating malnourished children.
In the two weeks after those supplies were not delivered, two children died of acute malnutrition.
The UN has warehouses in Damascus full of food aid, and Madaya is less than an hour’s drive away.
“The people that we are losing have families, they have children,” Dr Ahmad Tarakji, president of the Syrian American Medical Society and co-author of the report, said.
Last month dozens of activist groups accused the UN of “capitulating” to the Assad regime on the issue of aid delivery, ABC News reported.